Last stages in escort aptamer preparation cover man-made variations of one’s truncated aptamer
Further nuclease stabilization is achieved by substitution of 2?-OCHstep 3 for 2?-OH at purine positions. As the 2?-OCH3 moiety is not compatible with current SELEX process enzymes, this alteration must occur during chemical synthesis following evolution of a specific aptamer sequence. Generally, most of the 2?-OH purines can be substituted without loss of binding activity. At some locations, purines cannot be substituted without loss of affinity. 阅读更多